Plasmid

Part:BBa_K3600007:Design

Designed by: yasmine koubaa   Group: iGEM20_EPFL   (2020-10-27)


prTRX2-yomScarlet-I-tADH1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 936
    Illegal EcoRI site found at 1666
    Illegal XbaI site found at 945
    Illegal XbaI site found at 1843
    Illegal SpeI site found at 2811
    Illegal PstI site found at 2820
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 936
    Illegal EcoRI site found at 1666
    Illegal SpeI site found at 2811
    Illegal PstI site found at 2820
    Illegal NotI site found at 924
    Illegal NotI site found at 2830
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 2413
    Illegal EcoRI site found at 936
    Illegal EcoRI site found at 1666
    Illegal BglII site found at 2284
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 936
    Illegal EcoRI site found at 1666
    Illegal XbaI site found at 945
    Illegal XbaI site found at 1843
    Illegal SpeI site found at 2811
    Illegal PstI site found at 2820
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 936
    Illegal EcoRI site found at 1666
    Illegal XbaI site found at 945
    Illegal XbaI site found at 1843
    Illegal SpeI site found at 2811
    Illegal PstI site found at 2820
    Illegal NgoMIV site found at 2768
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

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Source

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References